Valacyclovir

Valacyclovir structural formula

CAS Number124832-26-4
Molecular FormulaC13H20N6O4
Molecular Weight324.342
InChI KeyHDOVUKNUBWVHOX-QMMMGPOBSA-N
LogP-0.521
Synonyms
  • Valacyclovir
  • 2-[(2-Amino-6-oxo-1,6-dihydro-9H-purin-9-yl)methoxy]ethyl L-valinate
  • L-Valine, 2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]ethyl ester
  • 124832-26-4
  • Valaciclovir
  • L-Valine ester with 9-((2-hydroxyethoxy)methyl)guanine
  • UNII-MZ1IW7Q79D
  • Valcyclovir
  • Val-ACV
  • Valcivir
  • L-Valine, 2-((2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy)ethyl ester

Applications:

HPLC Separation of Antiviral Drugs on Primesep 100 Column

April 1, 2021

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Oseltamivir, Valacyclovir


Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections.
Acyclovir, Valacyclovir, and Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.



Condition

Column Primesep 100, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer Gradient H2SO4 – 0.05- 0.8%, 10 min
Flow Rate 0.5 ml/min
Detection UV, 255 nm

 

Description

Class of Compounds
Drug
Analyzing Compounds Acyclovir, Valacyclovir, Oseltamivir

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Acyclovir
Oseltamivir
Valacyclovir
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Valacyclovir on Primesep 100 Column

March 31, 2021

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method for Analysis of Valacyclovir



Valacyclovir belongs to a class of drugs called antiviral drugs. Valacyclovir is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes as well as shingles. It can also be used to treat chickenpox in children. Valacyclovir can be retained on a Primesep 100 mixed-mode column with great peak shape using an isocratic method of acetonitrile (ACN), water and sulphuric acid (H2SO4) as a buffer. UV Detection 255nm.



Condition

Column Primesep 100, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 255 nm

 

Description

Class of Compounds
Drug
Analyzing Compounds Valaciclovir

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Valacyclovir
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Valacyclovir on Newcrom R1 HPLC column

February 16, 2018
Separation of Valacyclovir on Newcrom C18 HPLC column

Valacyclovir can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Valacyclovir
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.