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Separation of Maleic Acid Separation of Diacid: Ion Exclusion mode
Application Analytes: Dicarboxylic Acids Fumaric Acid Maleic Acid Malic Acid Succinic Acid Application Detection: UV Detection Separation of Diacid Hydrophobic and Ion Exclusion Modes  Primesep 200 retains and separates the organic diacids (malic, succinic, fumaric, and maleic) by a combination hydrophobic, reversed-phase interactions and ion exclusion. The separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Dicarboxylic Acids Fumaric Acid Maleic Acid Malic Acid Succinic Acid Application Detection: UV Detection Separation of Diacid Hydrophobic and Ion Exchange Modes  Primesep B combines a hydrophobic, reversed-phase mechanism with ion exchange to separate the diacids, fumaric, benzoic, phthalic, naphthoic, and maleic acids. Changing the acetonitrile content of the mobile phase reverses the peak order for naphthoic and maleic acids. Primesep B combines reversed-phase and anion-exchange mechanism with a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and UV detection at 250 nm. Application Analytes: Benzoic Acid Dicarboxylic Acids Fumaric Acid Maleic Acid Naphthoic Acid Phthalic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep A Column  Primesep A separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep 100 Column  Primesep 100 retains and separates an acid, base, and neutral in one HPLC injection. Maleic acid, benzylamine, and benzonitrile are resolved by ion-exclusion, ion-exhange and reversed-phase modes. The separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep 200 Column  Primesep 200 separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection Neutral, Base, and Acid on Primesep B Column  Primesep B separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. Application Analytes: Benzonitrile Benzylamine Maleic Acid Application Detection: UV Detection HPLC Analysis of Polar Basic and Acetic Compounds on Primesep AB Column
Application Analytes: Benzoic Acid Dopamine Maleic Acid Tyrosine Application Detection: UV Detection HPLC Separation of Organics Acids  Primesep D separates organic acids such as fumaric, benzoic, phthalic, naphthoic, and maleic acids by a mixture of anion exchange and reversed phase. Retention times and elution order can be changed by adjusting the percentage of acetonitrile in the mobile. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Application Analytes: Benzoic Acid Fumaric Acid Maleic Acid Naphthoic Acid Phthalic Acid Application Detection: UV Detection HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography  Majority of the drugs in pharmaceutical industries are administered in a salt form. The presence of two counter-ions very often requires two methods. The nature of these counterparts in drugs can be: inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Based on the property of molecules the stoichiometry can be different also. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by reversed-phase mechanism, and counter-ion are retained by reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing amount of acetonitrile and amount of ions in the mobile phase. Detection technique depends on the properties of counter-ions. In case of low or no UV activity, ELSD can be employed if counter-ion forms non-volatile salt wit mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. Presence of two mechanisms of retention allows to control retention times of drug and counter-ion independently, and even change order of elution when necessary. Application Analytes: Citric Acid Bromide Chloride Chlorate Perchlorate Maleic Acid Fumaric Acid Tartaric Acid Succinic Acid Phosphoric Acid Dextromethorphan Verapamil Pyrilamine Benzenesulfonic Acid p-Toluenesulfonic Acid (PTSA) Application Detection: UV Detection ELSD/MS Detection Separation of Maleic and Succinic Acid on Primesep B2 column
Organic and inorganic acids can be retained and separated on mixed-mode columns based on weak reversed-phase and weak/medium anion-exchange mechanisms. Amount of ACN, buffer concentration and buffer pH will affect retention time of organic and inorganic acids. Acids can be monitored by low UV, ELSD or LC/MS. Presence of ions is required to facilitate ion-exchange mechanism. Method can be used as a general approach for analysis of acidic hydrophilic and acidic hydrophobic compounds. Carboxylic acids along with inorganic acid can be retained and separated without ion-pairing reagent. Application Analytes: Maleic Acid Succinic Acid Application Detection: UV Detection ELSD/MS Detection HPLC Separation of Maleic Acid and Pentaerythritol
Application Notes: Maleic acid is a small hydrophilic acid, which is poorly retained in reversed-phase chromatography. Pentaerythritol is hydrophilic neutral molecule. A miixed-mode HILIC/anion-exchange method was developed on Obelisc N HPLC column. The retention time for maleic acid can be controlled by amount of ACN, buffer pH, and buffer concentration. Pentaerythritol retention time is only controlled by the amount of acetonitrile. The method is LC/MS and ELSD compatible. Application Analytes: Maleic Acid Pentaerythritol Application Detection: |
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