EDTA (Ethylenediaminetetraacetic Acid)

CAS Number 60-00-4
Molecular Formula C10H16N2O8
Molecular Weight 292.244 g/mol
InChI Key KCXVZYZYPLLWCC-UHFFFAOYSA-N
LogP -1.74
Synonyms
  • Ethylenediaminetetraacetic acid
  • 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo)tetraacetic acid
  • 60-00-4
  • Edetic acid
  • Glycine, N,N'-1,2-ethanediylbis[N-(carboxymethyl)-
  • EDTA
  • 1,2-Bis (N,N-dicarboxymethylamino)-ethane
  • 3,6-Diazaoctanedioic acid, 3,6-bis(carboxymethyl)-
  • Acetic acid, (ethylenedinitrilo)tetra-
  • Acetic acid, 2,2',2'',2'''-(1,2-ethanediyldinitrilo)tetrakis-
  • Acide edetique
  • acido edetico
  • Acroma DH 700
  • AETHYLENDIAMIN-TETRAESSIGSAEURE
  • Celon A
  • Celon ATH
  • Cheelox
  • Chemcolox 340
  • Clewat TAA
  • Clewat TTA
  • Complexon II
  • Dissolvine E
  • Dissolvine Z
  • DOHTITE 4H, EDTA ACID
  • Edathamil
  • EDETA B
  • Edetinsaure
  • EDTA ACID FORM
  • Endrate
  • ETHYLENE DINITRILO TETRAACETIC ACID
  • Ethylenediamine Tetra-Acetic Acid
  • Ethylenediamine-N,N,N',N'-tetraacetic acid
  • Ethylenediaminetetracetic acid
  • ETHYLENEDINITRILOTETRAACETIC ACID
  • Ethylene-N,N'-biscarboxymethyl-N,N'-diglycine
  • Gluma Cleanser
  • GLYCINE, N,N'-1,2-ETHANEDIYLBIS(N-(CARBOXYMETHYL))
  • Glycine,N,N'-1,2-ethanediylbis[N-(carboxymethyl)-,
  • Havidote
  • Metaquest A
  • N,N'-1,2-Ethanediylbis[N-(carboxymethyl)glycine]
  • N,N'-1,2-Ethanediyl-bis-N-(carboxymethyl)glycine
  • Nervanaid B acid
  • NSC 97243
  • NSC 97404
  • Nullapon B acid
  • Nullapon BF acid
  • Perma Kleer 50 acid
  • Quastal Special
  • Sequestrene AA
  • Sequestrene K 4
  • Sequestric acid
  • Sequestrol
  • Techrun DO
  • Titriplex
  • Titriplex II
  • Trilon BS
  • Trilon BW
  • Versene
  • Zonon AO
  • BRN 1716295
  • Caswell No. 438
  • Cheelox BF acid
  • EDTA acid
  • EINECS 200-449-4
  • EPA Pesticide Chemical Code 039101
  • Ethylenebisiminodiacetic acid
  • (Ethylenedinitrilo)tetraacetic acid
  • Hamp-ene acid
  • Komplexon II
  • Tetrine acid
  • Universne acid
  • Versene acid
  • Vinkeil 100
  • Warkeelate acid
  • Acide ethylenediaminetetracetique
  • Acidum edeticum
  • Kyselina ethylendiamintetraoctova
  • UNII-9G34HU7RV0
  • Ethylenebis(iminodiacetic acid)
  • 13440-78-3
  • 161122-33-4
  • 20539-27-9
  • 26627-46-3
  • 30485-87-1
  • 30485-88-2
  • 30485-90-6
  • 32757-10-1
  • 402925-67-1
  • 675141-16-9
  • 94108-75-5

Applications:


HPLC Application for Analysis of EDTA



EDTA (Ethylenediaminetetraacetic acid) is amino acid based compound which is widely used in industrial cleaners, detergents, photography, agrochemicals, textile, and food industries. EDTA serves as preservative in packaged food, vitamins and pharmaceutical formulations. It is used in organic and analytical laboratories as scavenger of metals, buffer solution, complexometric titration, masking agent for metal determination, etc.
EDTA is very polar compound with strong chelating properties. Low UV activity and strong binding to metal ions makes HPLC analysis of EDTA very difficult. Reproducible method for determination of wide range on concentration of EDTA in various formulations is developed using Primesep D anion-exchange mixed mode column. Copper sulfate is used for visualization purposes to increase sensitivity of the method. EDTA forms UV active complex with some metal ions. This method can be used for highly accurate quantitation of EDTA in different mixtures and composition. Method demonstrates controllable retention and perfect peak shape. Equilibration of the column prior to analysis requires special attention. 

If multiple injections produce different retention times, your column might not be equilibrated properly. Primesep D columns have a large ionic capacity towards anions. The shipping solvent for the Primesep D columns is ACN/water/TFA. The end point of gradient has only 4 mmol of sulfate ions. It takes over 5 hours to equilibrate a column with such low concentration. Before running your experiments you need to wash your column with 20% ACN and 0.2% of sulfuric acid for 1 hour. After you replace previous buffer/additive, you can set up your experiment. If you don’t change ionic modifier in your mobile phase the equilibration time is equivalent of  4-5 column volumes.



Application Analytes:

EDTA (Ethylenediaminetetraacetic Acid)

HPLC Retention of EDTA in Sulfated CD


EDTA, or Ethylenediaminetetraacetic acid, is widely used in textile, pulp/paper, food and pharmaceutical industries. It has application as chelating agent and preservative. EDTA molecule is very hydrophilic and contains two basic groups and four carboxylic groups. It has tendency to bind to metals, making analysis of EDTA by HPLC very challenging. EDTA is analyzed in the presence of copper sulfate as visualization agent on a Primesep SB column. The method can be used for EDTA determination in various mixtures and compositions using UV detector.



Application Analytes:

EDTA (Ethylenediaminetetraacetic Acid)

HILIC Separation of Common Preservatives - Citric Acid, Ascorbic Acid and EDTA



Citric acid, ascorbic acid and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.



Application Analytes:

Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C