HPLC Determination of Dopamine in Serum

FlipLC™ is an alternative method to avoid the interference of most of the contaminants by the use of an isolation column and a high pressure switching valve before the separation column. This method allows sample cleaning and analyte separation in one automated process. The isolation column and the separation column should have orthogonal retention characteristics to operate efficiently in this setup. Mixed-mode columns with reverse phase and ion-exchange characteristics were used in this analysis.


Column Primesep 200, 4.6x150 mm, 5 µm, 100A Primesep SB, 4.6*50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate  1.0 ml/min
Detection UV, 280 nm


Class of Compounds Drug, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Dopamine

Application Analytes:

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