HPLC Method for Analysis of Choline on Obelisc R Column

Choline is a water-soluble vitamin-like essential nutrient. It is a constituent of lecithin, which is present in many plants and animal organs. Choline is a rising star nutrient that is related to the B-vitamin family. First discovered in 1862, during the presidency of Abraham Lincoln and the first run of the hipster beard, it wasn’t classified as an essential nutrient until very recently.  

Condition

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 4.0- 5 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase
 

Description

Class of Compounds Drug,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Choline
 

Application Analytes:

Choline
Choline hydroxide