Primesep N

Primesep N is a normal-phase analytical column with embedded acidic groups.

  • Primesep N has embedded acidic groups with a pKa of about 5.
  • Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
  • Separates polar compounds by HILIC mechanism.
  • All mobile phases are LC-MS and preparative chromatography compatible.

Primesep N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 300 A, 100 A


Primesep N Application Examples

Effect of both pH and Organic Content on a Separation of Sugars, Amino

Acids, and Carboxylic Acids

HILIC Retention of Uridine and Uracil on Sielc’s HILIC Columns

HILIC Separation of Ritonavir and Ribavirin

HPLC Analysis of Pseudoephedrine and Citric Acid on Primesep Column

HPLC Derivatization and Quantitative Analysis of Glycerol

HPLC Method for Separation of Urea and Hydrogen Peroxide

HPLC Separation of Acetic acid and Acetic Hydrazide

HPLC Separation of Aromatic Diamine Disulfate

HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography

HPLC Separation of Heparines and Pentosan

HPLC Separation of Lecithin and Soy lecithin on Primesep N Column

HPLC Separation of Morpholino Sulfates

HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column

HPLC Separation of Saccharin and Sorbitol

HPLC Separation of Sulfamic and Sulfuric Acid on Primesep N HILIC Column

HPLC Separation of Uridine and Uracil

Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions