Primesep N is a normal-phase analytical column with embedded acidic groups.
- Primesep N has embedded acidic groups with a pKa of about 5.
- Improves retention of basic compounds by cation-exchange mechanism at pH > 5.
- Separates polar compounds by HILIC mechanism.
- All mobile phases are LC-MS and preparative chromatography compatible.
Primesep N columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 um, 5 um
Pores: 300 A, 100 A
Primesep N Application Examples
Effect of both pH and Organic Content on a Separation of Sugars, Amino
Acids, and Carboxylic Acids
HILIC Retention of Uridine and Uracil on Sielc’s HILIC Columns
HILIC Separation of Ritonavir and Ribavirin
HPLC Analysis of Pseudoephedrine and Citric Acid on Primesep Column
HPLC Derivatization and Quantitative Analysis of Glycerol
HPLC Method for Separation of Urea and Hydrogen Peroxide
HPLC Separation of Acetic acid and Acetic Hydrazide
HPLC Separation of Aromatic Diamine Disulfate
HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography
HPLC Separation of Heparines and Pentosan
HPLC Separation of Lecithin and Soy lecithin on Primesep N Column
HPLC Separation of Morpholino Sulfates
HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column
HPLC Separation of Saccharin and Sorbitol
HPLC Separation of Sulfamic and Sulfuric Acid on Primesep N HILIC Column
HPLC Separation of Uridine and Uracil
Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions