Primesep SB is a reverse-phase analytical column with strong embedded basic ion-pairing groups.
It contains a C18 carbon chain and acidic groups with pKa of 14.
- Improves retention of acidic compounds by anion-exchange mechanism.
- Separates bases by ion-exclusion mechanism.
- Retains neutral compounds by reverse-phase mechanism.
- All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 1.5 to 5).
Primesep SB columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 10 µm, 5 µm, 3 µm, 2.7 µm (solid core)
Pores: 100 A
Primsep SB Application Examples
HPLC Separation of Denatonium benzoate on Primesep SB Column
HPLC Determination of Vanillin on Primesep SB Column
HPLC Determination of Fosfomycin
HPLC Method for Analysis of Ascorbic acid and Sodium metabisulfite
HPLC Analysis of ACE Serum Active Ingredients
HPLC Analysis of Hop Extract (Beer Brewing)
HPLC Analysis of L-Cysteine-S-Sulfate on Primesep SB Column
HPLC Retention of EDTA in Sulfated CD
HPLC Retention of Ethylglucuronide
HPLC Retention of Sulfamic Acid on Primesep SB Mixed-Mode Column
HPLC Separation of 2-HEP and 2-HPP in the Presence of Methylcobalamin and Amino Acids
HPLC Separation of 5-Methyltetrahydrofolic Acid and Degradation Products
HPLC Separation of Alendronic, Ibandronic, Etidronic Acids
HPLC Separation of Ascorbic and Dehydroascorbic Acids
HPLC Separation of Epinephrine and Epinephrine Sulfonate
HPLC Separation of Methylphosphonic Acid and Related Products
HPLC Separation of Sodium, Chloride, Bromide, and Iodine
HPLC Separation of Sugar Phosphates
Separation of Nine Nucleotides by Mixed-Mode Chromatography
Separation of Nucleotide Monophosphates