Legacy L1

The Legacy L1 column is a silica-based, single-mode reversed-phase C₁₈column that meets the USP qualifications for the L1 designation. Compounds are retained based on their hydrophobic interactions with the ligands. The chromatograms in this section, as well as the others, show injections done according to USP standard methods for the given compounds, with modifications to the methods noted where applicable.

Legacy L1 columns are available in all standard dimensions.
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A

If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.

Prednisolone is an artificial corticosteroid that is used to treat many chronic ailments, such as colitis, multiple scelerosis, and arthritis. Figure 1 shows retention of Prednisolone on a Legacy L1 column with a mobile phase (MP) of 30% Acetonitrile (MeCN) and 70% water.

Epinephrine, or adrenaline, is a naturally-occurring corticosteroid. In the pharmaceutical industry, it is used to treat severe allergic reactions, heart attacks, and asthma. Figure 2 shows retention of Epinephrine on a Legacy L1 column with a mobile phase mostly consisting of methanol (MeOH) and water, with NaH2PO4, Sodium 1-Octanesulfonate, and EDTA as buffers.

Ketoprofen is a nonsteroidal anti-inflammatory drug (NSAID) used frequently to help treat arthritis-related pain and gum inflammation caused by severe toothaches. Figure 3 shows retention of Ketoprofen on a Legacy L1 column with a mobile phase consisting of water and acetonitrile and a high concentration of monosodium phosphate (MSP, NaH₂PO₄).

Amoxicillin is common antibacterial drug derived from penicillin. Figure 4 shows retention of Amoxicillin on a Legacy L1 column with a mobile phase consisting of methanol and mostly water with ammonium acetate as the buffer.

Legacy L1 column meets the USP definition with a stationary phase that consists of octadecyl silane chemically bonded to porous silica or ceramic particles (1.5 to 10µm).

Legacy L1 columns are available in all standard dimensions.
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A

If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.

Legacy L1

Legacy L1 column meets the USP definition with a stationary phase that consists of octadecyl silane chemically bonded to porous silica or ceramic particles (1.5 to 10µm).

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